FGF2 anticorps

N° du produit ABIN115676

L’anticorps Lapin Polyclonal anti-FGF2 a été validé pour WB et Func. Il convient pour détecter FGF2 dans des échantillons de Humain.

Aperçu rapide pour FGF2 anticorps (ABIN115676)

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FGF2 est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func)

Détail du produit anti-FGF2 anticorps

Specificité: The antibody will detect FGF-2 (basic).

Purification: Protein A affinity chromatography

Immunogène: Highly pure (> 98%) recombinant human FGF-2 (basic)

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human FGF-2 basic(5 ng/mL) on HUVEC cells, a concentration of about 3.5 μg/mL of this antibody is required. Western Blot: To detect human FGF-2 basic by Western blot this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant human FGF-2 (basic) is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of > / = 0.2 mg/mL.

Buffer: PBS, pH 7.4, without stabilizer or preservative

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > / = 0.2 mg/ml the antibody is stable for six weeks at 2-8 °C.

Date de péremption: 12 months

Détails sur FGF2

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Autre désignation: FGF Basic / FGF2

Sujet: FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2

ID gène: 2247

NCBI Accession: NP_001997

UniProt: P09038

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins